| Routinely used technologies in molecular biology and protein chemistry are available at the VRC, including (but not limited to): cloning, sequencing, RT-PCR, Southern and Northern blotting, immunoblotting, immunoprecipitation, flow cytometry, labeling and biotinylation of proteins (antibodies), reporter studies (luciferase and others), cell transfection, recombinant protein expression and purification (E. coli, Pichia pastoris, HEK293 cells, baculoviral methods), ELISA, plasmon resonance (Biacore), two-hybrid screens, library screening, BAC recombineering, RACE PCR, genome walking, scintillation proximity assay, gateway cloning, analytical methods (HPLC, dHPLC), antibody production and upscaling, etc.
Apart from these standard technologies, the VRC also provides service in bioinformatics, antibody phage display to generate our own antibodies (library containing 1010 different sequences from D. Neri, ETH Zurich), and has proprietary libraries of morpholinos (4,500 oligomers), zebrafish cDNA library (containing > 50,000 sequence tags), tadpole cDNA library (containing > 5,800 different gene products of which 800 bps of 5’ sequence) that failitate the design of knockdown strategies in these small aquatic animal models.
Several knock-out mouse strains are available for collaborations. Feel free to contact
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for more information. |
 
Automated colony picking
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Core Facilities 
